Effects of pH and agar on in vitro embryo culture of orange cv. Pêra

Authors

  • Valtemir Gonçalves Ribeiro
  • Moacir Pasqual
  • José Darlan Ramos
  • Arnaldo Freitas de Oliveira Junior
  • Gladyston Rodrigues Carvalho

DOI:

https://doi.org/10.1590/S1678-3921.pab1997.v32.4760

Keywords:

Citrus sinensis, citriculture, embryo culture, breeding

Abstract

This work had the objective of determining the effects of pH and agar interaction on in vitro orange (Citrus sinensis Osb.) embryo culture. The 0; 3.5; 7.0; 10.5 and 14.0 g/L agar concentrations were associated with MS media at 3.7; 4.7; 5.7 and 6.7 pH values, previously sterilized in autoclave. The 4.7 pH value showed higher shoot and root growth. Higher percentage of plant survival was achieved at pH 6.7 at 10.5 g/L agar concentration and a linear decrease in plant fresh weight was observed as agar concentration increased.

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Published

1997-11-01

How to Cite

Ribeiro, V. G., Pasqual, M., Ramos, J. D., de Oliveira Junior, A. F., & Carvalho, G. R. (1997). Effects of pH and agar on <i>in vitro</i> embryo culture of orange cv. Pêra. Pesquisa Agropecuaria Brasileira, 32(11), 1147–1152. https://doi.org/10.1590/S1678-3921.pab1997.v32.4760

Issue

v.32, n.11, nov. 1997

Section

POMOLOGY