Propagation of orchid Gongora quinquenervis by in vitro germination

Abstract

The aim of this experiment was to study in vitro germination and organogenesis of Gongora quinquenervis in two culture media (Knudson "C" and Murashige & Skoog) on three concentrations of BAP (0.0, 0.5 and 1.0 mg L^-1). The protocorms cultured on Knudson "C" medium died. The majority of the embryos cultured on the Murashige & Skoog medium formed calli. The calli presented a high morfogenetic potential, regenerating great number of plant through indirect organogenesis, and the treatment without BAP presented the highest regeneration frequency. Few plants were germinated through the normal route, performing a total of 41 plants, which contrasts with the 715 plants regenerated through indirect organogenesis. The aim of this experiment was to study in vitro germination and organogenesis of Gongora quinquenervis in two culture media (Knudson "C" and Murashige & Skoog) on three concentrations of BAP (0.0, 0.5 and 1.0 mg L-1). The protocorms cultured on Knudson "C" medium died. The majority of the embryos cultured on the Murashige & Skoog medium formed calli. The calli presented a high morfogenetic potential, regenerating great number of plant through indirect organogenesis, and the treatment without BAP presented the highest regeneration frequency. Few plants were germinated through the normal route, performing a total of 41 plants, which contrasts with the 715 plants regenerated through indirect organogenesis.