Selection and characterization of Bacillus thuringiensis strains effective to control the diamondback moth Plutella xylostella

Authors

  • Patrícia Teles Medeiros Universidade Federal de Mato Grosso,
  • Márcio do Nascimento Ferreira Universidade Federal de Mato Grosso
  • Érica Soares Martins Embrapa Recursos Genéticos e Biotecnologia
  • Ana Cristina Menezes Mendes Gomes Embrapa Recursos Genéticos e Biotecnologia
  • Rosana Falcão Embrapa Recursos Genéticos e Biotecnologia
  • José Manoel Cabral de Souza Dias Embrapa Recursos Genéticos e Biotecnologia
  • Rose Gomes Monnerat Embrapa Recursos Genéticos e Biotecnologia

DOI:

https://doi.org/10.1590/S1678-3921.pab2005.v40.7077

Keywords:

protein cristals, entomopathogenic bacteria, biopesticides

Abstract

The aim of this work was to select and characterize the most toxic Bacillus thuringiensis strains, from the Germplasm Bank of Bacillus spp. of Empresa Brasileira de Pesquisa Agropecuária, against Plutella xylostella. Strains were characterized by morphological, biochemical and molecular methods. It was observed that seven out of the 203 strains tested showed high toxicity compared to the standard used B. thuringiensis subsp. kurstaki (HD-1), which showed 100% mortality. Selected strains showed features described for lepidoptera regarding the protein of 130 kDa and 65 kDa; profile and features were obtained through the PCR reactions, making possible to identify the presence of cry1 and cry2 genes. Moreover, the scanning electron microscopy showed the bipiramydal, cubed and round crystal forms. The selected strains offer new perspectives to control P. xylostella.

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Published

2005-11-01

How to Cite

Medeiros, P. T., Ferreira, M. do N., Martins, Érica S., Gomes, A. C. M. M., Falcão, R., Dias, J. M. C. de S., & Monnerat, R. G. (2005). Selection and characterization of <i>Bacillus thuringiensis</i> strains effective to control the diamondback moth <i>Plutella xylostella</i>. Pesquisa Agropecuaria Brasileira, 40(11), 1145–1148. https://doi.org/10.1590/S1678-3921.pab2005.v40.7077

Issue

v.40, n.11, nov. 2005

Section

SCIENTIFIC NOTES