Validation of molecular markers associated to leaf rust resistance genes in wheat

Authors

  • Paulo Roberto Da Silva Universidade Estadual do Centro-Oeste
  • Sandra Cristina Kothe Milach Pioneer Sementes Ltda
  • Vinicius de Albuquerque Sortica UFRGS
  • Tatiana Boff UFRGS
  • Sandra Patussi Brammer Embrapa Trigo
  • Luiz Carlos Federizzi UFRGS

DOI:

https://doi.org/10.1590/S1678-3921.pab2008.v43.727

Keywords:

Puccinia triticina, Triticum aestivum, SCAR, STS

Abstract

The objective of this work was to validate molecular markers previously associated to leaf rust resistance genes in Brazilian wheat genotypes. Five STS and SCAR markers identifi ed as associated to the
resistance alleles of the genes Lr1, Lr9, Lr10 and Lr24 were tested by PCR in 25 Brazilian wheat genotypes with known presence or absence of these alleles. The STS marker associated to Lr1 was not effi cient in identifying
Brazilian genotypes carrying its resistance allele. The STS and SCAR markers associated to Lr9, Lr10 and Lr24 are effi cient in identifying plants carrying the resistance alleles for these genes, and therefore, can be used
for marker-assisted selection of leaf rust resistance in Brazilian wheat genotypes.

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Published

2008-10-29

How to Cite

Silva, P. R. D., Milach, S. C. K., Sortica, V. de A., Boff, T., Brammer, S. P., & Federizzi, L. C. (2008). Validation of molecular markers associated to leaf rust resistance genes in wheat. Pesquisa Agropecuaria Brasileira, 43(10), 1357–1363. https://doi.org/10.1590/S1678-3921.pab2008.v43.727

Issue

v.43, n.10, out. 2008

Section

GENETICS