GUS gene expression driven by a citrus promoter in transgenic tobacco and 'Valencia' sweet orange

Authors

  • Fernando Alves de Azevedo Centro APTA Citros “Sylvio Moreira”
  • Francisco de Assis Alves Mourão Filho Escola Superior de Agricultura Luiz de Queiroz
  • Evandro Henrique Schinor Escola Superior de Agricultura Luiz de Queiroz
  • Luis Gustavo de Paoli Escola Superior de Agricultura Luiz de Queiroz
  • Beatriz Madalena Januzzi Mendes Centro de Energia Nuclear na Agricultura
  • Ricardo Harakava Instituto Biológico
  • Dean W. Gabriel University of Florida
  • Richard F. Lee USDA-ARS National Clonal Germplasm Repository for Citrus and Dates

DOI:

https://doi.org/10.1590/S1678-3921.pab2006.v41.7325

Keywords:

Xylella fastidiosa, PAL, citrus variegated chlorosis, cultivar resistance

Abstract

The objective of this work was the transformation of tobacco and 'Valencia' sweet orange with the GUS gene driven by the citrus phenylalanine ammonia-lyase (PAL) gene promoter (CsPP). Transformation was accomplished by co-cultivation of tobacco and 'Valência' sweet orange explants with Agrobacterium tumefaciens containing the binary vector CsPP-GUS/2201. After plant transformation and regeneration, histochemical analyses using GUS staining revealed that CsPP promoter preferentially, but not exclusively, conferred gene expression in xylem tissues of tobacco. Weaker GUS staining was also detected throughout the petiole region in tobacco and citrus CsPP transgenic plants.

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Published

2006-11-01

How to Cite

de Azevedo, F. A., Mourão Filho, F. de A. A., Schinor, E. H., de Paoli, L. G., Mendes, B. M. J., Harakava, R., … Lee, R. F. (2006). GUS gene expression driven by a citrus promoter in transgenic tobacco and ’Valencia’ sweet orange. Pesquisa Agropecuaria Brasileira, 41(11), 1623–1628. https://doi.org/10.1590/S1678-3921.pab2006.v41.7325

Issue

v.41, n.11, nov. 2006

Section

GENETICS