Alternaria brown spot resistant citrus hybrid identification by means of fAFLP and pathogenicity tests

Authors

  • Edvan Alves Chagas
  • Jairo Osvaldo Cazetta
  • Eliana Gertrudes Macedo Lemos
  • Moacir Pasqual
  • Antonio Goes
  • Jose Darlan Ramos
  • Rafael Pio
  • Wilson Barbosa
  • Vander Mendonça
  • Luis Alberto Ambrosio

DOI:

https://doi.org/10.1590/S1678-3921.pab2007.v42.7655

Keywords:

Citrus sinensis, Citrus reticulata, genetic improvement, plant biotechnology, molecular markers

Abstract

The objective of this work was to identify hybrids obtained from controlled crossings between 'Folha Murcha' x 'Ponkan', and to check their resistance to Alternaria alternata f. sp. citri. The seedlings were obtained by in vitro embryo culture. The fAFLP marker technique was used to identify the hybrids, then laboratory pathogenicity test of the hybrids was accomplished with Alternaria alternata f. sp. citri isolates. The pairs of primers EcoRI AAG – Msel CAG and EcoRI ACC – Msel CAA were the most efficient for hybrids identification, and distinguished 48.5% of hybrids. Hybrids F64, F108, F111, F113, F131 and F139 showed potential resistance to the Alternaria alternata f. sp. citri.

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Published

2007-07-01

How to Cite

Chagas, E. A., Cazetta, J. O., Lemos, E. G. M., Pasqual, M., Goes, A., Ramos, J. D., … Ambrosio, L. A. (2007). Alternaria brown spot resistant citrus hybrid identification by means of fAFLP and pathogenicity tests. Pesquisa Agropecuaria Brasileira, 42(7), 975–983. https://doi.org/10.1590/S1678-3921.pab2007.v42.7655

Issue

v.42, n.7, jul. 2007

Section

POMOLOGY