Micropropagation methods of pineapple

Abstract

The objective of this work was to compare different methods of pineapple in vitro mass micropropagation. The treatments were conventional micropropagation in solid medium (5 and 6 g L^-1 agar) and in liquid medium, micropropagation in bioreactor of temporary immersion (immersion in culture medium each 2, 4 and 8 hours, for 3 minutes) and continuous immersion (with aeration each 2, 4 and 8 hours, for 3 minutes). MS medium supplement with 1 mg L^-1 BAP, 0.25 mg L^-1 NAA, and pH adjusted to 5.8 was utilized. Cultures were incubated at 25±1^oC under cool white fluorescent tubes (40 µmol m^-2 s^-1) with a 16-hours photoperiod. The experiment design was completely randomized, comprising nine treatments and four replicates. After 45 days, number of shootings, shootings higher than 1 cm, length of shootings, fresh matter of shootings and dry matter of shootings were evaluated. Temporary immersion system in each 2 hours for 3 minutes provided higher number, height and dry matter of pineapple shootings. Temporary immersion system is the most efficient method in pineapple mass micropropagation.