Genetic transformation of 'Valencia' sweet orange with the cecropin MB39 gene

Authors

  • Luis Gustavo de Paoli
  • Raquel Luciana Boscariol Camargo
  • Ricardo Harakava
  • Beatriz Madalena Januzzi Mendes
  • Francisco de Assis Alves Mourão Filho

DOI:

https://doi.org/10.1590/S1678-3921.pab2007.v42.7746

Keywords:

Agrobacterium tumefaciens, Citrus sinensis, CVC, antibacterial peptide, xylem

Abstract

The objective of this work was to produce 'Valencia' sweet orange transgenic plants with the cecropin MB39 gene controlled by a phenylalanine ammonia-lyase gene promoter from citrus in order to direct gene expression in xylem vessels. The genetic transformation was mediated by Agrobacterium tumefaciens with the co-culture of epicotyl segments. Eleven transgenic plants were selected by PCR with the amplification of a 189 bp fragment, which were acclimatized to greenhouse. The integration of the transgene was confirmed in three plants by Southern blot analysis.

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Published

2007-11-01

How to Cite

Paoli, L. G. de, Camargo, R. L. B., Harakava, R., Mendes, B. M. J., & Filho, F. de A. A. M. (2007). Genetic transformation of ’Valencia’ sweet orange with the <i>cecropin</i> MB39 gene. Pesquisa Agropecuaria Brasileira, 42(11), 1663–1666. https://doi.org/10.1590/S1678-3921.pab2007.v42.7746

Issue

v.42, n.11, nov. 2007

Section

SCIENTIFIC NOTES