fAFLP markers to characterize three mume genotypes selected as rootstocks for peach tree

Abstract

The objective of this work was the identification of fAFLP markers to be used in molecular characterization of three mume genotypes selected as rootstocks for peach tree. Twenty-four different fAFLP primer combinations were used and allowed the recognition of 648 markers, comprising 272 markers which were able to discriminate the three clones one from the other. These markers were used to calculate the groupment of the clones according to their similarities with the distance method and neighbour joining algorithm. The same markers were also used to calculate the genetic distance among the clones. The fAFLP markers were efficient to identify the clones, mainly by the combinations of selective primers Fam ACT/CAT, Joe AGG/CTT and Ned AGC/CAA. fAFLP markers allowed the genetic discrimination of the three mume genotypes. The greatest genetic distance was found between Rigitano cultivar and Clone 15. The fAFLP markers also showed greater genetic similarity between Clone 05 and Rigitano cultivar.