Induced-feeding bioassays for detection of Bacillus thuringiensis insecticidal activity against Epilachna paenulata (Coleoptera)

Authors

  • Diego Herman Sauka Instituto Nacional de Tecnología Agropecuaria, Instituto de Microbiología y Zoología Agrícola, Insumos Bacterianos, De los Reseros y Las Cabañas
  • Rodrigo Horacio Monella Instituto Nacional de Tecnología Agropecuaria, Instituto de Microbiología y Zoología Agrícola, Insumos Bacterianos, De los Reseros y Las Cabañas
  • Graciela Beatriz Benintende Instituto Nacional de Tecnología Agropecuaria, Instituto de Microbiología y Zoología Agrícola, Insumos Bacterianos, De los Reseros y Las Cabañas

DOI:

https://doi.org/10.1590/S1678-3921.pab2010.v45.7823

Keywords:

biological control, Coccinellidae, Cucurbitaceae, ladybird beetle, spore-crystal toxicity

Abstract

The objective of this work was to establish and test the induced-feeding bioassay in order to detect Bacillus thuringiensis insecticidal activity against (Coleoptera: Coccinellidae). Larvae were induced to swallow high concentrations of spore-crystal suspensions of s Argentine B. thuringiensis strains. The great majority of strains showed no toxicity, and observed mortality was lower than 30%. Induced-feeding bioassay is feasible, and should be used for prospecting strains that produce right combinations of Cry proteins needed to an efficient pest control.

Author Biography

Diego Herman Sauka, Instituto Nacional de Tecnología Agropecuaria, Instituto de Microbiología y Zoología Agrícola, Insumos Bacterianos, De los Reseros y Las Cabañas

Investigador

Instituto de Microbiología y Zoología Agrícola, INTA

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Published

2011-01-18

How to Cite

Sauka, D. H., Monella, R. H., & Benintende, G. B. (2011). Induced-feeding bioassays for detection of <i>Bacillus thuringiensis</i> insecticidal activity against <i>Epilachna paenulata</i> (Coleoptera). Pesquisa Agropecuaria Brasileira, 45(4), 430–432. https://doi.org/10.1590/S1678-3921.pab2010.v45.7823

Issue

v.45, n.4, abr. 2010

Section

SCIENTIFIC NOTES