Organogenesis of the leaf explant of Eucalyptus grandis x E. urophylla clones

Abstract

The aim of this work was to evaluate the effects of growth regulators TDZ [1-phenil-3-(1,2,3-thiadiazol-5-yl)urea], BAP (6-benzilaminopurine) e NAA (Naphthalene acetic acid) on the in vitro propagation by organogenesis from foliar explants of Eucalyptus grandis x E. urophylla. Depending on the clone used, there were singular responses to growth regulators treatment regarding callusing intensity, texture and color. The best results of the three genotypes used were observed with the TDZ (0.5 mg L^-1) and NAA (0.1 mg L^-1) treatment, where 100% of the foliar explants presented callus. The worst results were observed with the BAP (0.1 mg L^-1) and NAA (0.1 mg L^-1) treatment. Subsequently, considering the regeneration process, the best response was achieved with 1.0 mg L^-1 BAP, in which 8% of the calli regenerated buds, with an average of 4.2 buds per explant.