Identification of the begomovirus resistance genes Ty‑2 and Ty‑3 in tomato genotypes

Authors

  • Jorge González Aguilera Universidade Federal de Viçosa
  • Francisco Dueñas Hurtado Instituto Nacional de Ciencias Agrícolas
  • Cesar Augusto Diniz Xavier Departamento de Fitopatologia
  • Bruno Soares Laurindo Departamento de Fitotecnia
  • Carlos Nick Departamento de Fitotecnia
  • Marta Álvarez Gil Instituto Nacional de Ciencias Agrícolas
  • Derly José Henriques da Silva Departamento de Fitotecnia
  • Francisco Murilo Zerbini Departamento de Fitopatologia

DOI:

https://doi.org/10.1590/S1678-3921.pab2011.v46.9936

Keywords:

Solanum lycopersicum, Solanum peruvianum, geminivirus, PCR, genetic resources

Abstract

The objective of this work was to identify the presence of the genes begomovirus resistence Ty‑2 and Ty‑3 in tomato accessions of the Banco de Germoplasma de Hortaliças of the Universidade Federal de Viçosa. The oligonucleotides TO302 F/R and FLUW25 F/R were used in PCR analysis to verify the presence of markers associated to the genes Ty‑2 and Ty‑3, respectively. The Ty‑2 gene was observed in heterozygosis in the accession BGH-6881 (Solanum peruvianum), and the Ty‑3 gene in homozygosis in the accessions BGH-6878, BGH-6897 (S. lycopersicum), and in heterozygosis in acession BGH-6881. The identification of resistance genes, using PCR analysis, represents an advance for tomato breeding programs in Brazil.

Author Biographies

Jorge González Aguilera, Universidade Federal de Viçosa

http://lattes.cnpq.br/8562342815666974

Cesar Augusto Diniz Xavier, Departamento de Fitopatologia

http://lattes.cnpq.br/9062524731229088

Bruno Soares Laurindo, Departamento de Fitotecnia

http://lattes.cnpq.br/5267666881711305

Carlos Nick, Departamento de Fitotecnia

http://lattes.cnpq.br/8999214264404271

Derly José Henriques da Silva, Departamento de Fitotecnia

http://lattes.cnpq.br/6243238937742884

Francisco Murilo Zerbini, Departamento de Fitopatologia

http://lattes.cnpq.br/6507826653693228

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Published

2011-09-02

How to Cite

Aguilera, J. G., Hurtado, F. D., Xavier, C. A. D., Laurindo, B. S., Nick, C., Gil, M. Álvarez, … Zerbini, F. M. (2011). Identification of the begomovirus resistance genes <i>Ty‑2<i/> and <i>Ty‑3</i> in tomato genotypes. Pesquisa Agropecuaria Brasileira, 46(7), 772–775. https://doi.org/10.1590/S1678-3921.pab2011.v46.9936

Issue

v.46, n.7, jul. 2011

Section

SCIENTIFIC NOTES