Obtaining a virus-free 'seleta folha murcha' though micrografting In vitro

Abstract

In this paper, the 'Seleta Folha Murcha', a probable variation of the citrus sinensis (L.) cv. Seleta do Rio, of great productivity and of late maturity is described. The results of in vitro micrografting utilization for a virus-free variety obtention are also reported. This technique is also utilized do get plants wihout the majority of virus-like diseases, and to maintain, at the same time, characteristics of the desired cultivar. The rootstock utilized was a 'Rangpur Lime' (citrus limonia Osbeck), obtained from seeds that where germinated in mineral salt culture medium with Bacto-agar, under constant darkness. The micrografted citrus obtained have already been immunized and are kept in the acclimatization phase in a greenhouse, where they will be indexed as for the main citrus virus, using test plants.