Isolation of rabies virus from kidney, heart and brain of naturally infected cow

Abstract

Rabies virus was isolated by intracerebral inoculation of baby and adult mice by using emulsions from kidney, heart and brain tissues of a naturally infected cow in the County of São Fidelis, State of Rio de Janeiro, Brazil. The virus strains isolated from different tissues were identified as rabies virus by microscopic examination of the brains of inoculated mice, revealing presence of Negri bodies in the citoplasm of nervous cells and by serum neutralization tests using standardized rabies serum.