Gene flow on insect‑resistant genetically modified maize

Authors

  • Vivian Elias Nascimento UFLA
  • Édila Vilela de Resende Von Pinho
  • Renzo Garcia Von Pinho
  • João Cândido de Souza
  • André Domingos do Nascimento Júnior

DOI:

https://doi.org/10.1590/S1678-3921.pab2012.v47.10822

Keywords:

Zea mays, outcrossing, coexistence norms, real‑time PCR, transgenic

Abstract

The objective of this work was to estimate the gene flow on insect‑resistant transgenic maize in grain production fields. Grain samples were collected in fields containing conventional and transgenic maize in the Brazilian municipalities of: Itumirim, Uberlândia, Paracatu, and Tupaciguara, in Minas Gerais State; Itapetininga and Pedrinhas, in São Paulo State; and Assaí and Ponta‑Grossa, in Paraná State. Seed samples were collected in conventional maize crops starting 5 m away from the transgenic maize source. Ten corn cobs were collected from individual plants per sampling point, with four replicates, totaling 40 corn cobs at each evaluated distance. Gene flow analyses were carried out by real‑time polymerase chain reaction (PCR). On average, 82% of cross‑fertilization occurred within the first 30 m. The highest outcrossing rates were observed in Itapetininga, SP, – at rates above 10% up to a distance of 50 m; however, less than 1% at 100 m distance. The 20-m isolation, with ten border lines, is not sufficient to ensure outcrossing rates lower than 1%.

Author Biographies

Vivian Elias Nascimento, UFLA

Édila Vilela de Resende Von Pinho

Renzo Garcia Von Pinho

João Cândido de Souza

André Domingos do Nascimento Júnior

Published

2012-07-13

How to Cite

Nascimento, V. E., Von Pinho, Édila V. de R., Von Pinho, R. G., Souza, J. C. de, & Nascimento Júnior, A. D. do. (2012). Gene flow on insect‑resistant genetically modified maize. Pesquisa Agropecuaria Brasileira, 47(6), 784–790. https://doi.org/10.1590/S1678-3921.pab2012.v47.10822

Issue

Section

CROP SCIENCE