Influence of culture media and growth periods on the sporulation Septoria glycines

Abstract

The object of the present study was to find a new culture medium to provide rapid and abundant Septoria glycines (Hemmi) sporulation, the causal agent of brown spot disease on soybean leaves. First, carbon and nitrogen sources and growth regulators were compared. Later, based on the first results obtained, the culture media used as checks, associated with growth factors in doses which resulted in the greatest spore production, were compared (Czapek agar and PDA). These were evaluated in five growth periods. In the first trials, the malt extract, yeast extract and thiamine growth factors were greater than the basic media. In the final trial, the treatments containing the yeast extract growth factor continued with the greatest spore production in the five different periods observed during 25 days. The greatest spore production was obtained when the culture reached 25 days of development, with the Czapek agar medium + 4 g/l of yeast extract.