Survival of sugarcane shoot tips after cryopreservation by droplet-vitrification

Authors

  • Gabriela Ferreira Nogueira Embrapa Recursos Genéticos e Biotecnologia
  • Moacir Pasqual Universidade Federal de Lavras, Departamento de Agricultura
  • Jonny Everson Scherwinski-Pereira Embrapa Recursos Genéticos e Biotecnologia

DOI:

https://doi.org/10.1590/S1678-3921.pab2013.v48.17488

Keywords:

Saccharum, in vitro conservation, PVS2, regeneration

Abstract

The objective of this work was to evaluate the phytotoxicity of a plant vitrification solution (PVS2), and the survival of shoot tips of the sugarcane variety SP716949, after cryopreservation by droplet‑vitrification. Shoot tips were precultured for 24 hours in MS medium containing 0.3 mol L-1 sucrose, and exposed to PVS2 for 0, 20 or 30 min. Shoot tips were then immersed in liquid nitrogen. Thawing was fast in concentrated sucrose solution (1.2 mol L-1). PVS2 is a nontoxic to shoot tips, which in turn are sensitive to liquid nitrogen. The best results occurred when shoot tips were maintained for up to 20 min in PVS2 solution, before freezing, with 20% survival.

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Published

2014-02-11

How to Cite

Nogueira, G. F., Pasqual, M., & Scherwinski-Pereira, J. E. (2014). Survival of sugarcane shoot tips after cryopreservation by droplet-vitrification. Pesquisa Agropecuaria Brasileira, 48(11), 1524–1527. https://doi.org/10.1590/S1678-3921.pab2013.v48.17488

Issue

v.48, n.11, nov. 2013

Section

SCIENTIFIC NOTES