Occurrence and molecular analysis of quarantine virus in lily cultivation areas in Brazil

Authors

  • Eliana Borges Rivas Instituto Biológico (IB), Centro de Pesquisa e Desenvolvimento de Sanidade Vegetal (CPDSV), Laboratório de Diagnóstico Fitopatológico, Avenida Conselheiro Rodrigues Alves, no 1.252, CEP 04014‑002 São Paulo, SP.
  • Estevão Cardoso de Almeida Bôdi Biólogo autonomo, Rua Esteves Júnior, no 545, CEP 88015‑130 Florianópolis, SC.
  • Ricardo Harakava Instituto Biológico (IB), Centro de Pesquisa e Desenvolvimento de Sanidade Vegetal (CPDSV), Laboratório de Bioquímica Fitopatológica, CEP 04014‑002 São Paulo, SP.
  • Fabio Gregori Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Laboratório de Biologia Molecular Aplicada e Sorologia, Avenida Professor Dr. Orlando Marques de Paiva, no 87, CEP 05508‑270 São Paulo, SP.
  • Marcos Cesar Gonçalves IB, CPDSV, Laboratório de Bioquímica Fitopatológica, CEP 04014‑002 São Paulo, SP.

DOI:

https://doi.org/10.1590/S1678-3921.pab2016.v51.20813

Keywords:

Lilium, Lily mottle virus, Lily symptomless virus, phylogeny, recombination analysis, RT‑PCR

Abstract

The objective of this work was to describe the occurrence of quarantine Tulip breaking virus (TBV, synonym Lily mottle virus – LMoV) and Lily symptomless virus (LSV), and their respective molecular analyses, to provide data for supporting TBV removal from the Brazilian A1 quarantine pest list, since this virus has spread among the main commercial lily crops in Brazil. The occurrence of these viruses was detected in 12 cultivation areas through multiplex reverse transcription (RT‑PCR), using specific primers to genes encoding the respective coat proteins (CP). Eight fragments of 800 nucleotides (nt) obtained from the LMoV‑infected lilies and nine fragments of 600 nt from LSV‑infected lilies were sequenced. Phylogenetic tree reconstruction showed a robust branch containing the LMoV Brazilian sequences, other LMoV isolates, TBV, and Tulip band breaking virus, suggesting that all are LMoV isolates, although they are clustered into two subgroups. Phylogenetic analysis also showed a robust branch supporting all Brazilian and other LSV sequences, except for an LSV Japanese isolate. Recombination analyses also showed an LMoV recombinant isolate, whereas no recombination events were found among LSV isolates. Lily mottle virus is the prevalent virus in lily crops in Brazil, in single and mixed infections with LSV or Cucumber mosaic virus (CMV).

Author Biographies

Eliana Borges Rivas, Instituto Biológico (IB), Centro de Pesquisa e Desenvolvimento de Sanidade Vegetal (CPDSV), Laboratório de Diagnóstico Fitopatológico, Avenida Conselheiro Rodrigues Alves, no 1.252, CEP 04014‑002 São Paulo, SP.


Estevão Cardoso de Almeida Bôdi, Biólogo autonomo, Rua Esteves Júnior, no 545, CEP 88015‑130 Florianópolis, SC.


Ricardo Harakava, Instituto Biológico (IB), Centro de Pesquisa e Desenvolvimento de Sanidade Vegetal (CPDSV), Laboratório de Bioquímica Fitopatológica, CEP 04014‑002 São Paulo, SP.


Fabio Gregori, Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Laboratório de Biologia Molecular Aplicada e Sorologia, Avenida Professor Dr. Orlando Marques de Paiva, no 87, CEP 05508‑270 São Paulo, SP.


Marcos Cesar Gonçalves, IB, CPDSV, Laboratório de Bioquímica Fitopatológica, CEP 04014‑002 São Paulo, SP.


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Published

2016-08-03

How to Cite

Rivas, E. B., Bôdi, E. C. de A., Harakava, R., Gregori, F., & Gonçalves, M. C. (2016). Occurrence and molecular analysis of quarantine virus in lily cultivation areas in Brazil. Pesquisa Agropecuaria Brasileira, 51(5), 615–622. https://doi.org/10.1590/S1678-3921.pab2016.v51.20813

Issue

v.51, n.5, maio 2016: Número Temático Pesquisa, Desenvolvimento e Inovações em Face de Ameaças Sanitárias para a Agropecuária

Section

PHYTOPATHOLOGY