Androgenic response of Brazilian wheat genotypes to different pretreatments of spikes and to a gelling agent

Authors

  • Liane Balvedi Poersch-Bortolon Universidade Federal do Rio Grande do Sul, Departamento de Genética, Avenida Bento Gonçalves, no 9.500, Caixa Postal 15.053, CEP 91501-970 Porto Alegre, RS, Brazil
  • Sandra Maria Mansur Scagliusi Embrapa Trigo, Rodovia BR-285, Km 294, Caixa Postal 3081, CEP 99050‑970 Passo Fundo, RS, Brazil
  • Elene Yamazaki-Lau Embrapa Trigo, Rodovia BR-285, Km 294, Caixa Postal 3081, CEP 99050‑970 Passo Fundo, RS, Brazil
  • Maria Helena Bodanese-Zanettini Universidade Federal do Rio Grande do Sul, Departamento de Genética, Avenida Bento Gonçalves, no 9.500, Caixa Postal 15.053, CEP 91501-970 Porto Alegre, RS, Brazil

DOI:

https://doi.org/10.1590/S1678-3921.pab2016.v51.21849

Keywords:

Triticum aestivum, albinism, androgenesis, doubled haploid, isolated microspore culture, recalcitrance

Abstract

The objective of this work was to analyze the androgenic response of Brazilian wheat genotypes to different pretreatments of the spikes, prior to the culture of isolated microspores, and to the effect of a gelling agent in the induction culture medium. Five genotypes were evaluated for embryo formation, green plant
regeneration, and spontaneous chromosome duplication. Wheat spikes were subjected to two pretreatments:cold, at 4°C for 21 days; and 2-hydroxynicotinic acid, at 32°C for two days. Culture media were evaluated with or without Ficoll as a gelling agent. Cold produced more embryos and green plants than the chemical pretreatment in four out of five genotypes. Only two genotypes treated with 2-hydroxynicotinic acid were able to produce plants, and one of them produced a single albino plant. Medium containing Ficoll produced more
embryos than liquid medium and promoted a higher number of plants. Spontaneous chromosome duplication varies between genotypes and pretreatments, and shows high variability.

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Published

2016-12-23

How to Cite

Poersch-Bortolon, L. B., Scagliusi, S. M. M., Yamazaki-Lau, E., & Bodanese-Zanettini, M. H. (2016). Androgenic response of Brazilian wheat genotypes to different pretreatments of spikes and to a gelling agent. Pesquisa Agropecuaria Brasileira, 51(11), 1839–1847. https://doi.org/10.1590/S1678-3921.pab2016.v51.21849

Issue

v.51, n.11, nov. 2016

Section

GENETICS