Contents of total phenolics and flavonoids in and antioxidant activity of Ilex paraguariensis leaves

Authors

  • Dilson Antônio Bisognin Universidade Federal de Santa Maria, Departamento de Fitotecnia, Avenida Roraima no 1.000, Cidade Universitária, Bairro Camobi, CEP 97105-900 Santa Maria, RS.
  • Leandro Vinícius da Luz Universidade Federal de Santa Maria, Programa de Pós-graduação em Engenharia Florestal, Avenida Roraima no 1000, Cidade Universitária, Bairro Camobi, CEP 97105-900 Santa Maria, RS.
  • Kelen Haygert Lencina Universidade Federal de Santa Maria, Programa de Pós-graduação em Engenharia Florestal, Avenida Roraima no 1000, Cidade Universitária, Bairro Camobi, CEP 97105-900 Santa Maria, RS.
  • Clarissa Obem dos Santos Universidade Federal de Santa Maria, Programa de Pós-graduação em Tecnologia de Alimentos, Avenida Roraima no 1000, Cidade Universitária, Bairro Camobi, CEP 97105-900 Santa Maria, RS.
  • Cláudia Kaehler Sautter Universidade Federal de Santa Maria, Departamento de Tecnologia e Ciência dos Alimentos, Avenida Roraima, no 1.000, Cidade Universitária, Bairro Camobi, CEP 97105-900 Santa Maria, RS.

DOI:

https://doi.org/10.1590/S1678-3921.pab2019.v54.26571

Keywords:

mate, polarity, polyphenols, solvents

Abstract

The objective of this work was to evaluate different solvents and times in the ultrasound-assisted extraction of polyphenols, in order to quantify the total phenolic and flavonoid contents in and the antioxidant activity of mate (Ilex paraguariensis) leaves. The experiment was carried out in a completely randomized design, in a 8x3 (solvent x time of extraction) factorial arrangement. The solvent extractors were distilled water and aqueous solutions of ethanol, acetone, and methanol, acidified or not with hydrochloric acid, and the time of ultrasound-assisted extractions were 15, 30, and 45 min. All extractions were finished with a mechanical agitation of 30 min. Samples treated with acidified hydroalcoholic solvents resulted in higher values of total phenolics and flavonoids and antioxidant activity. The extraction using acidified hydroethanol solvent was not affected by the time in the ultrasound. The contents of total phenolics and flavonoids were highly correlated with each other and with the antioxidant activity, especially when it was determined by the ferric-reducing antioxidant power method. Acidified hydroethanol solvent in ultrasound for 15 min is the most suitable extraction condition for the quantification of the contents of total phenolics and flavonoids in and the antioxidant activity of mate leaves.

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Published

2019-09-25

How to Cite

Bisognin, D. A., Luz, L. V. da, Lencina, K. H., Santos, C. O. dos, & Sautter, C. K. (2019). Contents of total phenolics and flavonoids in and antioxidant activity of <I>Ilex paraguariensis</I> leaves. Pesquisa Agropecuaria Brasileira, 54(X), e0856. https://doi.org/10.1590/S1678-3921.pab2019.v54.26571