Promotion of plant growth and rooting of stem cuttings by endophytic bacteria from black pepper roots

Abstract

The objective of this work was to investigate three endophytic bacterial strains as to their ability to promote the growth and rooting of black pepper stem cuttings, as well as to determine the mechanisms involved in the promoting activity through molecular methods. The strains were identified by 16S sequencing as belonging to the genera Bacillus, Priestia, and Lysinibacillus. They were characterized for the production of indoleacetic acid (IAA), phosphate solubilization, and siderophore production. In two assays, the roots of the cuttings were immersed in bacterial suspensions to evaluate growth promotion through plant height, stem diameter, and root and shoot dry mass. The Bacillus sp. C1.4 and Priestia sp. T2.2 strains were able to produce siderophores, whereas Priestia sp. T2.2 and Lysinibacillus sp. C5.11 produced IAA. In addition, Priestia sp. T2.2 significantly increased plant height and dry mass, whereas Lysinibacillus sp. C5.11 significantly increased root dry mass. Therefore, Priestia sp. T2.2 and Lysinibacillus sp. C5.11 are able to promote the growth and rooting of black pepper stem cuttings, respectively. This growth promotion is linked directly to the production of IAA and siderophores.