Ascorbyl palmitate and tocopherol acetate as metabolic antioxidants in dourado larvae

Abstract

The objective of this work was to evaluate the effect and interaction between supplementation of tocopherol acetate and ascorbyl palmitate, in the diet of dourado larvae (Salminus brasiliensis), during its
initial development. The experimental design was completely randomized in split plot, as follows: in the plot, a factorial arrangement (2x3) with six diets, constituted by the combination between two concentrations of
tocopherol acetate (0 and 250 mg kg-1) and three concentrations of ascorbyl palmitate (0, 100 and 1,000 mg kg-1); and in the subplot, with two periods of sampling (5 and 15 days of feeding). In the larvae, determinations were performed for the concentrations of the ascorbyl palmitate, ascorbic acid and dehydroascorbic acid, total length,
weight and height of the head. Ascorbyl palmitate provided an increase in the total length and in the weight of the larvae after 15 days of feeding. For head height, differences were observed among the three doses of tested ascorbyl palmitate. The supplementation of ascorbyl palmitate increased the vitamin C concentrations. Although
vitamin E has not infl uenced the development sizes, it acted as a metabolic pro-oxidant, which increased the
dehydroascorbic acid.