Plant regeneration from embryogenic and organogenic calli in oat

Authors

  • Fernanda Bered
  • Maria Jane Cruz de Melo Sereno
  • Fernando Irajá Félix de Carvalho
  • Claúdia Erna Lange
  • Cristine Luise Handel
  • Ana Lúcia Cunha Dornelles

DOI:

https://doi.org/10.1590/S1678-3921.pab1998.v33.5017

Keywords:

in vitro regeneration, tissue culture, somatic embryogenesis

Abstract

Nine oat (Avena sativa L.) genotypes were evaluated aiming to test in vitro regeneration ability. Immature embryos between 1 and 3 mm were inoculated in MS medium supplemented with 4 mg/L of 2,4-D. In the next stage, 2,4-D was reduced, and then removed at the plant regeneration stage. The obtained calli were tested for somatic embryoids production. The correlation between this character and the regeneration was tested. Plant regeneration was observed for all genotypes; however, variability was detected for this character. The embryogenesis x regeneration analysis revealed low correlation between these characters, showing that plant regeneration may have occurred preferentially via organogenesis and germination.

Published

1998-11-01

How to Cite

Bered, F., Cruz de Melo Sereno, M. J., Félix de Carvalho, F. I., Erna Lange, C., Luise Handel, C., & Cunha Dornelles, A. L. (1998). Plant regeneration from embryogenic and organogenic calli in oat. Pesquisa Agropecuaria Brasileira, 33(11), 1827–1833. https://doi.org/10.1590/S1678-3921.pab1998.v33.5017

Issue

Section

PLANT PHYSIOLOGY