Adventitious shoot of ornamental pineapple under benzylaminopurine, naphthalene acetic acid and subculture period effect

Abstract

The objective of this work was to evaluate the effect of benzylaminopurine (BAP) additions, in combination with naphthaleneacetic acid (NAA), and of subcultures periods on in vitro shoot formation of ornamental pineapple. The basal liquid medium consisted of MS salts and vitamins. The experiment was a completely randomized design, in a factorial arrangement 6x2x4, with six concentrations of BAP (0, 0.125, 0.25, 0.5, 1, e 2 mg L-1) and two of NAA (0 and 0.1 mg L-1), and four subculturing periods (30, 60, 90 e
120 days). The explants consisted of individualized shoots with 1 cm length from in vitro cultures. Shoot formation was observed in medium supplemented with BAP in all subculturing periods. The greatest average
number of shoots per explant was obtained with BAP concentration of 1.2 mg L-1 and 120 days of subcultive.